SDS-MW Determination

The SDS-MW Analysis Kit is designed for the separation of protein-SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective sieving range of approximately 10 kDa to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility. So the molecular weight of an unknown protein may be estimated from a standard curve of known protein sizes. This kit can also be used to quantify the amount of protein and to determine the purity of a protein product. (from Application Guide to SDS-MW Analysis Kit, Beckman Coulter, Inc.)

See the documents below for specific details about sample preparation and use of instrument.

SDS-MW Background Information

Beckman SDSMW_A51970 document

 

Updated: 1/8/14